mutation screening in the mitochondrial d-loop region of tumoral and non-tumoral breast cancer in iranian patients
نویسندگان
چکیده
the mitochondrial dna (mtdna) mutations in mitochondrial coding and non coding regions seem to be important in carcinogenesis. the aim of this investigation was to evaluate coding region (mt-trnaphe and trnapro) and non-coding sequence, mitochondrial displacement loop (mtdna d-loop), in the cancerous and non-cancerous lesions of iranian patients with breast cancer (bc). genomic dna was extracted from 50 breast tumors and surrounding normal tissue pairs as well as from 50 unrelated normal breast tissues from iranian kurdish population. subsequently, pcr amplification was performed using specific primers, and then pcr products were subjected to direct sequencing. 41 genetic variants were identified in mtdna d-loop among tumoral and non-tumoral tissues but not in trnaphe and trnapro sequences. our findings indicated that c182t, 194inst, 285insa and 16342delt were just found in bc tumors whereas 302insc, c309t and c16069t found in both tumors and surrounding normal tissues. although our findings showed that the observed genetic variations were not restricted to breast cancer tissues, some genetic changes were found only in bc tumors. our results, in agreement with the evidence from earlier studies, confirm that the mtdna genetic alterations might be implicated in tumor initiation, progression and development. text-align: justify;
منابع مشابه
Mutation screening in the mitochondrial D-loop region of tumoral and non-tumoral breast cancer in Iranian patients.
The mitochondrial DNA (mtDNA) mutations in mitochondrial coding and non coding regions seem to be important in carcinogenesis. The aim of this investigation was to evaluate coding region (mt-tRNA(Phe) and tRNA(Pro)) and non-coding sequence, mitochondrial displacement loop (mtDNA D-loop), in the cancerous and non-cancerous lesions of Iranian patients with breast cancer (BC). Genomic DNA was extr...
متن کاملthe study of aaag repeat polymorphism in promoter of errg gene and its association with the risk of breast cancer in isfahan region
چکیده: سرطان پستان دومین عامل مرگ مرتبط با سرطان در خانم ها است. از آنجا که سرطان پستان یک تومور وابسته به هورمون است، می تواند توسط وضعیت هورمون های استروئیدی شامل استروژن و پروژسترون تنظیم شود. استروژن نقش مهمی در توسعه و پیشرفت سرطان پستان ایفا می کند و تاثیر خود را روی بیان ژن های هدف از طریق گیرنده های استروژن اعمال می کند. اما گروه دیگری از گیرنده های هسته ای به نام گیرنده های مرتبط به ا...
15 صفحه اولthe investigation of research articles in applied linguistics: convergence and divergence in iranian elt context
چکیده ندارد.
the u.s. policy in central asia and its impact on the colored revolutions in the region (the case study of tulip revolution in kyrgyzstan)
چکیده ندارد.
15 صفحه اول"inmunhistochemical Determination of Telomerase Expression in Tumoral and Non Tumoral Breast Tissue"
Telomerase is a DNA polymerase RNA dependent enzyme that adds telomere sequences at the end of the chromosomes compensating the loss of telomeres during cellular proliferation. Recent studies have reported a correlation between telomerase and clinicopathologic characteristics of certain tumors, suggesting a possible role as a future prognostic marker. Most of studies however, have detected leve...
متن کاملProteomic analysis of non-tumoral breast tissue.
Breast cancer is a complex and heterogeneous disease. In spite of the advances made in recent decades, a better understanding of the intrinsic mechanisms of this disease is crucial. The development of new biomarkers is absolutely necessary to improve diagnosis and prognosis. Research using the proteomic approach has generated interesting results; however, the complexity of the mammary glan...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
acta medica iranicaجلد ۵۰، شماره ۷، صفحات ۴۴۷-۴۵۳
کلمات کلیدی
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023